Go Back   SEQanswers > Sequencing Technologies/Companies > Ion Torrent

Similar Threads
Thread Thread Starter Forum Replies Last Post
cancer panel on miseq bartekd Illumina/Solexa 3 01-29-2013 12:56 PM
Early experiences with 316 chips nickloman Ion Torrent 15 06-25-2012 08:17 PM
200bp kit with 316 chip + question: larger fragments selection Gorbenzer Ion Torrent 1 06-08-2012 09:13 AM
Ion Torrent 316 Chip Sale!!!! Brand New Unopened dnathrowaway Ion Torrent 21 02-13-2012 11:46 AM
314 and 316 with 200pb ION Torrent kant1_IC Ion Torrent 1 01-19-2012 01:40 AM

Thread Tools
Old 08-01-2013, 04:02 PM   #1
Senior Member
Location: chicago

Join Date: Jul 2012
Posts: 353
Default 316 and panel

The panel is 20 genes, 923 amplicons, target size 86kb, coverage 98.26%. The problem is when 2 samples are barcoded the run does not look good and the off-target reads are high. When the same panel in run just one sample at a time the run looks good and the off-target reads are low. Any thoughts or sugesstions. Thanks.
cmccabe is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 10:06 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2019, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO