Go Back   SEQanswers > Sequencing Technologies/Companies > Sanger/Dye Terminator

Similar Threads
Thread Thread Starter Forum Replies Last Post
Redirecting ABySS temp directory thh32 Bioinformatics 4 03-18-2014 06:42 AM
End-repair at wrong temp vonlogan Sample Prep / Library Generation 0 08-21-2013 05:16 AM
min-coverage-normal & min-coverage-tumor in VarScan somatic Jane M Bioinformatics 2 11-20-2012 08:46 AM
Bisulfite sequencing - filtering by min. conversion rate mixter Epigenetics 3 06-09-2012 01:33 AM
PE bisulfite seq: could the EB solution inhibit the PCR rxn? Simone78 Illumina/Solexa 6 03-25-2011 06:15 AM

Thread Tools
Old 03-19-2014, 06:09 PM   #1
Junior Member
Location: Southern California

Join Date: Aug 2013
Posts: 8
Default Sequencing rxn left at room temp for ~45 min

Hi y'all, I left my complete sequencing reaction at room temp right outside the thermocycler by accident for about ~45 min b/c I was waiting for the lid to heat and forgot about it. It's the normal Big Dye terminator sequencing rxn, forward or reverse primer, big dye, water, sequencing buffer. I found my mistake and quickly put it into the PCR machine. DO you think it will be ok? I do. Of course BDTv3.1 is light sensitive but it was kinda in the shade, not under direct lights.. I hope 45 min isn't too long. Thanks!
FredTam1 is offline   Reply With Quote

big dye, sequencing reaction, troubleshooting

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 08:04 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2019, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO