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Old 03-19-2014, 06:09 PM   #1
FredTam1
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Location: Southern California

Join Date: Aug 2013
Posts: 8
Default Sequencing rxn left at room temp for ~45 min

Hi y'all, I left my complete sequencing reaction at room temp right outside the thermocycler by accident for about ~45 min b/c I was waiting for the lid to heat and forgot about it. It's the normal Big Dye terminator sequencing rxn, forward or reverse primer, big dye, water, sequencing buffer. I found my mistake and quickly put it into the PCR machine. DO you think it will be ok? I do. Of course BDTv3.1 is light sensitive but it was kinda in the shade, not under direct lights.. I hope 45 min isn't too long. Thanks!
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