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Old 09-15-2015, 11:57 PM   #1
Location: Eesti

Join Date: Jan 2009
Posts: 37
Default Illumina amplicon sequencing and singletons

hi SEQanswers,

I have a question about ssu rRNA gene fragment analysis when the amplicons are sequenced on NGS. In old times when we had 454 data, a singleton was considered to be 1 unique sequence. When I do now Illumine PE sequencing, I have the lowest number of reads 3, non of 2 or 1 unique sequences. Why it is so?

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