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Old 11-02-2011, 05:14 AM   #1
arnaud.kr
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Default Bisulphite sequencing - Ion Torrent

Hello

Does anyone has an experience with sequencing of bisulphite converted DNA on Ion-torrent ? Is there any conceptual reason why this would not work ?
Thanks in advance

Arnaud
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Old 11-11-2011, 03:06 AM   #2
svz
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Hi, Arnaud
We tried to sequence bisulphite DNA (PCR fragments). It works. But some troubles occurred with long stretches such as TTTT. Sometimes ion torrent sequenced it as TTT or TTTT or TTTTT or TTTTTT.
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