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Old 11-08-2011, 01:44 PM   #1
Cramsmith
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Default Formulation of Low TE

So I can't find the formulation/concentration of the Low TE that comes with the Ion kits anyone know what the concentration and pH of the Tris-EDTA should be?
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Old 11-09-2011, 12:04 PM   #2
cliffbeall
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Low TE generally is 10 mM Tris pH 8 0.1 mM EDTA, don't know for sure if Ion Torrent uses that.
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Old 11-10-2011, 07:46 AM   #3
Cramsmith
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Quote:
Originally Posted by cliffbeall View Post
Low TE generally is 10 mM Tris pH 8 0.1 mM EDTA, don't know for sure if Ion Torrent uses that.
Thanks, that is what I was assuming
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Old 12-05-2011, 05:35 AM   #4
julihepple
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Hi there

Is this Low TE then acceptable to use for diluting gDNA in the Ion Shear fragment Xpress kit? I did an ethanol precipitation and re suspended the gDNA in RNase-free dH2O to be safe but I would prefer to use it as is, if it wont affect the reaction.

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Old 12-08-2011, 01:44 PM   #5
Cramsmith
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Yes, I would use this buffer to resuspend your DNAs.
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