SEQanswers

Go Back   SEQanswers > Sequencing Technologies/Companies > Ion Torrent



Similar Threads
Thread Thread Starter Forum Replies Last Post
SR Adapters on a PE flowcell? andibody Illumina/Solexa 15 09-17-2014 05:38 AM
SOLiD Adapters exprofiler SOLiD 36 10-30-2012 11:04 PM
Clipping adapters nsl RNA Sequencing 6 05-27-2011 02:08 PM
adapters avatar10 General 2 12-02-2010 05:04 AM
Solexa adapters Paedhaemdoc General 14 11-19-2009 06:46 AM

Reply
 
Thread Tools
Old 02-01-2012, 02:30 AM   #1
Chartiza
Junior Member
 
Location: Moscow

Join Date: Jun 2010
Posts: 8
Default Adapters sequence

Hi all!
Can't find the adaptors sequence for Amplicon Sequencing)) Can smb help me?

thanks!)))
Chartiza is offline   Reply With Quote
Old 02-01-2012, 02:35 AM   #2
ulz_peter
Senior Member
 
Location: Graz, Austria

Join Date: Feb 2010
Posts: 219
Default

maybe you should start specifying which technology you're using on which instrument...
ulz_peter is offline   Reply With Quote
Old 02-01-2012, 02:38 AM   #3
Chartiza
Junior Member
 
Location: Moscow

Join Date: Jun 2010
Posts: 8
Default

Ion Torrent, Amplicon sequencing))
Chartiza is offline   Reply With Quote
Old 02-01-2012, 02:39 AM   #4
TonyBrooks
Senior Member
 
Location: London

Join Date: Jun 2009
Posts: 298
Default

Assuming it's Ion Torrent as it's in the Ion Torrent thread...
Attached Files
File Type: pdf amplicon_application_note_IonTorrent.pdf (1.14 MB, 638 views)
TonyBrooks is offline   Reply With Quote
Old 02-01-2012, 02:45 AM   #5
Chartiza
Junior Member
 
Location: Moscow

Join Date: Jun 2010
Posts: 8
Default

In this manual are only the primer sequence, ... but adaptor sequence absence? is they full complementary and same size?

Last edited by Chartiza; 02-01-2012 at 02:53 AM.
Chartiza is offline   Reply With Quote
Old 02-01-2012, 02:57 AM   #6
TonyBrooks
Senior Member
 
Location: London

Join Date: Jun 2009
Posts: 298
Default

The PCR creates the adapter sequences using the P1 and A tags on the site specific primers.
TonyBrooks is offline   Reply With Quote
Old 02-01-2012, 06:15 AM   #7
DICER
Member
 
Location: Northern hemisphere

Join Date: Jan 2012
Posts: 12
Default

having the sequence of the (Y) adaptors would be handy!
DICER is offline   Reply With Quote
Old 02-01-2012, 08:01 AM   #8
TonyBrooks
Senior Member
 
Location: London

Join Date: Jun 2009
Posts: 298
Default

What's interesting is that these sequences seem to be a mash up of the 454 and Solid sequences. There is even some part complimentary sequence between the forward and reverse primer sequences. Surely this is would promote primer-dimer?

http://seqanswers.com/forums/showthread.php?t=16450
TonyBrooks is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 11:12 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2019, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO