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Old 06-27-2012, 12:59 PM   #1
jffkm
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Default Bridge amplification: yield per cluster

I'm wondering if anyone has data on the approximate yield for a single cluster during bridge amplification (i.e. ng/cluster for a theoretical 100bp target). In the context of liquid PCR I imagine it's limited due to physical contraints vs. diffusion but if anyone has data on this, I'd be really interested. thanks!
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Old 06-28-2012, 09:38 AM   #2
cliffbeall
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This Illumina document (PDF) says "up to 1000 identical molecules" of each DNA per cluster, so sub-femtogram.

Last edited by cliffbeall; 06-28-2012 at 09:40 AM. Reason: grammar
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Old 06-28-2012, 09:45 AM   #3
jffkm
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Default Thanks!

Thanks for the prompt reply! Exactly the info I needed.
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