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Old 11-28-2011, 12:00 AM   #1
singaporeseq
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Default Multiplexing with ion torrent?

I am looking for a protocol or personal experience in doing multiplexing with ion torrent. Has anybody done this?
The app note is not very informative
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Old 11-28-2011, 06:16 AM   #2
krobison
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What sort of samples are you trying to multiplex? It makes a big difference.

For amplicons, one route is to just design appropriate tags (ones designed for the 454 will work) into your primers.

For fragment libraries, NuGen has a kit and I believe Ion has their own similar kits.
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Old 11-28-2011, 06:38 AM   #3
singaporeseq
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Thanks for the info. I was interested in both amplicons and also bacterial whole genome.
I wanted to see if anyone has experience with the ion barcode kits. I can't find much information on them.
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Old 11-30-2011, 07:49 PM   #4
ionman
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Have you seen the Ion Express gDNA and Amplicon Library preparation guide posted on the Ion Community?
http://lifetech-it.hosted.jivesoftwa.../docs/DOC-1997
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