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Old 09-24-2012, 05:18 AM   #21
pmiguel
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Originally Posted by Vinz View Post
BD V1.1 is supposed to be better for the short products than BD V3.1
We use POP6 on one of our 3730XL for reading early. Using BD V1.1 and Pop6 reading from base 20 onwards is no problem. If you correct it manually, you could read from base 1 often. Caveats: Pop6 is only sold in very small aliquots and with a huge price tag.
Ah, I remember when we bought it in big (100 ml?) bottles for the 3700 -- $300 each. What a shame for Applied Biosystems that manufacturing POP-6 is so expensive that they have been forced to increase their prices by nearly three orders of magnitude. I am sure they are weeping bitter tears over that.

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If you are interested I could share the settings.
Please post them here.

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Phillip
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Old 09-24-2012, 05:27 AM   #22
Vinz
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There is an alternative source for the POP6:
http://www.mclab.com/NanoPOP-TM-Polymers.html
However, so far we did not take the effort and risk of testing if those perform similar...
If you test those we would be interested in your experience.
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Old 09-25-2012, 04:54 AM   #23
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Yep, I heard about those, too. I haven't heard anything bad about them. The company apparently regenerate arrays after they "go bad".
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