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Old 07-25-2012, 12:52 PM   #1
uberfinch
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Location: boston

Join Date: Dec 2011
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Default How do Nextera and TSCA have the same chemistry?

Hi everyone,


I'm puzzled: on the samplesheet for Nextera libraries, the chemistry is listed as "amplicon", yet we know that the transposase sequence at the 3' end of the adapters is going to require a different forward read primer. How does the MiSeq (which is the platform I'm using) distinguish between these two? The generated sample sheets seem nearly identical. Perhaps they just make a mix of the two primers together?

I'm interested in the answer for my own custom amplicon application. I know others have posted their approaches but I need to do something slightly different, but want the dual indexing.

Thanks for insights you can provide!

Evan
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