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Old 09-15-2015, 10:38 PM   #1
jinu chirayath
Junior Member
 
Location: INDIA

Join Date: Jan 2015
Posts: 1
Question BLAST + database creation for plant microRNA s from miRBase

Hello all,

I have installed NCBI-Blast-2.2.28 on win-7 32 bit system .
Also set the path variable to bin directory
i want to create database of plant microRNAs
i have downloaded mature micoRNAs from miRBase
Right now i am having an excel file containing plant microRNas from miRBase
I want to convert this data in to blastable format
I have converted the excel file into csv format and then in to fasta format
and tried makeblastdb command , but it outputs BLAST options errorr: file doesn't exist
can you please help me regarding this

Thanks for your help in advance
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