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Old 11-27-2018, 11:17 AM   #1
carlos aylwin
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Location: Oregon

Join Date: Jan 2018
Posts: 1
Default Circular chromatin conformation capture

Hi Community,
I'm starting working with 4C libraries and not sure if Ligation reaction worked or not. After digestion with the first restriction enzyme, I could see the decrease in the MW pattern by agarose gel. However, that pattern did not change after ligation (6 days in total). I used the de Laat Protocol published in Methods in Enzymology, 2012.
Should large circular DNA fragments be seen in the gel after ligation? Let me know your experiences. Thanks
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