SEQanswers

Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation



Similar Threads
Thread Thread Starter Forum Replies Last Post
RNase treatment wormseq Sample Prep / Library Generation 4 09-06-2013 12:13 AM

Reply
 
Thread Tools
Old 12-14-2018, 07:48 AM   #1
drdoodlebug
Junior Member
 
Location: Austin, Texas

Join Date: May 2012
Posts: 5
Default RNase treatment before NGS metagenomic community profiling? Yea or nay?

I get asked this question all the time and never no how to respond. May one of y'all knows the answer. If a person is preparing DNA, is it necessary to perform RNAse treatment prior to amplifying the 16S gene (say V4 to run on miseq)? Asked another way: will the presence of RNA interfere with or skew the community profile of DNA used for NGS metagenomic community profiling?
drdoodlebug is offline   Reply With Quote
Old 12-15-2018, 01:33 AM   #2
nucacidhunter
Jafar Jabbari
 
Location: Melbourne

Join Date: Jan 2013
Posts: 1,210
Default

For PCR based amplification of 16S variable regions RNase treatment usually is not necessary. It will impact correct quantification by spectrophotometric methods if it is included in the workflow. If the same extracted DNA will be used for other applications as well it is better to include RNase in one of extraction steps.
nucacidhunter is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 06:46 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2019, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO