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Old 12-15-2018, 04:54 AM   #1
Junior Member
Location: Hannover

Join Date: Sep 2018
Posts: 3
Default Correct Ratio for two-sided PCR-cleanup with Ampure XP beads

Hey guys,
I want to use Ampure XP beads for size selection after PCR. I tried to find the right ratio of beads : pcr-product but got mixed results so far.

I'm curious, what ratios should work approximately for a two-sided clean-up if i want to select pcr-products around 170-230bp.

I would like to have a very clean product. The PCR is run with genomic DNA which has been sheared to a length of around 10000bp and the Primers used are around 55bp. Both should be cleaned out as well as possible.

Thanks for the input.
bheida is offline   Reply With Quote
Old 12-15-2018, 11:00 PM   #2
Jafar Jabbari
Location: Melbourne

Join Date: Jan 2013
Posts: 1,210

Double SPRI size selection window will be much larger than 50 bp. For your target you need to use Sage Pippin or gel extraction.
nucacidhunter is offline   Reply With Quote
Old 01-10-2019, 01:01 AM   #3
Location: Aarhus, Denmark

Join Date: Mar 2008
Posts: 52

Take a look at
But I agree with nucacidhunter that 50 bp is a too narrow window for a double-SPRI
JakobHedegaard is offline   Reply With Quote

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