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Old 02-14-2019, 09:43 AM   #1
thiNGS
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Location: Netherlands

Join Date: Sep 2014
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Default Inhibitors of DNA-magnetic beads binding

Hi all! I am trying to optimize cfDNA extraction testing different kits with commercial samples (artificial plasma). For some reason one set of samples does not work well with the magnetic beads kit. What do you think is inhibiting the binding? pH? Some chemicals present in the commercial samples? I recover 10x less the estimated amount so there must be some incompatibility... Thanks in advance.
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