Tweet
bacterial wgs library loading on MiSeq v3 600 cycles
Just wondering how much wgs library (prepared by Nextera xt kit) should be loaded on MiSeq using v3 600 cycle kit. We tried two times, 20 pM and 14 pM, both runs yielded only ~7 gb (half of 15 gb). We did several amplicon libraries which were pretty good. Are there any tricks? Thank you very much in advance.
-
-
Your experience indicates issues with correct quantification and loading. For good yield cluster density should be over 1200k/mm2 and this can be achieved with18-19 pM loading.
For specific diagnosis you can contact Tech support at Illumine or post more information about library (trace), quantification method, if bead normalisation was used and run summary page from SAV.
-
The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...05-06-2024, 07:48 AM -
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM
Comment