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Old 09-26-2017, 02:49 PM   #1
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Location: California, SF bayarea

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Default PCR bias in library construction

I am thinking of investigating PCR bias in NGS library preps. I have seen a review and a few articles detailing bias with different polymerases. Kappa hi-fi was mentioned as having the lowest bias. I just wondered if there was a consensus for those who are in the trenches doing the experiments and if anyone could point me to further research on the subject.

Thank you.
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pcr bias

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