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Old 02-19-2016, 05:44 AM   #1
genefuse
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Question Tn5

Does anyone know of another source of Tn5 transposase other than
FC 121 1030 form Illumina.
Cheers
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Old 02-19-2016, 06:13 AM   #2
Olaf Blue
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Epicentre's EZ-Tn5 Transposase; TNP92110, which is a special order item.
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Old 02-19-2016, 07:34 AM   #3
genefuse
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Thank you for the suggestion. Can Epicentre's EZ-Tn5 TNP92110 completely substitute for the Illumina product in terms of primer sequence, buffers, reaction conditions, etc.
Appreciated,
Genefuse
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Old 02-19-2016, 07:37 AM   #4
Olaf Blue
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Happy to help - this is just the bare enzyme and users have to load any oligos themselves. Here's the product page link:

http://www.epibio.com/enzymes/in-viv...posase?details

Reaction buffer and stop solution is provided; we don't do custom oligo loading.

Disclaimer: I am part of ILMN in Madison, WI
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Old 02-19-2016, 07:42 AM   #5
genefuse
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Great!
Much appreciated
Genefuse
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Old 10-13-2017, 12:31 AM   #6
irene_chen
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Hi, gene fuse
Have you tried the EZ-Tn5 from epicenter? Could it be used for DNA library preparation?
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Old 10-13-2017, 07:15 AM   #7
genefuse
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Default Tn5

Hi,
I have not had a chance to try EN-Tn5.
Olaf Blue might have more info on this
Genefuse
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Old 10-13-2017, 07:16 AM   #8
Olaf Blue
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EZ-Tn5 from Epicentre has been transferred for sales and support to Lucigen:

http://www.lucigen.com/EZ-Tn5-Transposase/

Olaf
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Old 10-13-2017, 07:25 AM   #9
irene_chen
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I am finding the replacement of Nextera from Illumina but I am not sure if it could be used for tagmentation. It seems to be mostly used for the gene mutation.
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