Hi
I have NGS exome sequencing results (fastq file) and I generated FASTQC report for it, and AFAICS the result looks like sequencing data is suboptimal. I am alien to the field, and would like to get community's opinion on how good/bad/typical the data actually is.
The lab told they used Ion Proton and followed ACMG standards - no more details, don't know chemistry kit.
The report below, and thanks in advance.
FASTQC version 0.11.2
File type: Conventional base calls
Encoding: Sanger / Illumina 1.9
Total Sequences: 33803990
Sequences flagged as poor quality: 0
Sequence length: 8-376
%GC: 48
Per base sequence quality, Error icon
Per sequence quality scores, Warning icon
Per base sequence content, Error icon
Per sequence GC content, Warning icon
Per base N content, OK icon
Sequence Length Distribution, Warning icon
Sequence Duplication Levels, OK icon
Overrepresented sequences, OK icon
No overrepresented sequences
Adapter Content, OK icon
Kmer Content, Error icon
I have NGS exome sequencing results (fastq file) and I generated FASTQC report for it, and AFAICS the result looks like sequencing data is suboptimal. I am alien to the field, and would like to get community's opinion on how good/bad/typical the data actually is.
The lab told they used Ion Proton and followed ACMG standards - no more details, don't know chemistry kit.
The report below, and thanks in advance.
FASTQC version 0.11.2
File type: Conventional base calls
Encoding: Sanger / Illumina 1.9
Total Sequences: 33803990
Sequences flagged as poor quality: 0
Sequence length: 8-376
%GC: 48
Per base sequence quality, Error icon
Per sequence quality scores, Warning icon
Per base sequence content, Error icon
Per sequence GC content, Warning icon
Per base N content, OK icon
Sequence Length Distribution, Warning icon
Sequence Duplication Levels, OK icon
Overrepresented sequences, OK icon
No overrepresented sequences
Adapter Content, OK icon
Kmer Content, Error icon
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