Hi there,
I am gonna work on gene expression profiling in a non-model plant species, my advisor suggested using ScriptSeqTM Complete Kit*(plant) for sequencing libraries construction and then do a paired end sequencing(Supposed to be better than single end sequencing for the purpose of de novo assemble ?) using illumina Hiseq-2000.
Without a reference genome, I guess I will need either to use ref genome from a close related species or do a de novo assemble.
According to the library construction protocol, there is no size selection step for the final sequencing library, though they suggested to use bioanalyzer to estimate the size distribution in the library. So, my question is: will it be necessary to add a size selection step (200~400bp?) before sending out the library for a paired-end sequencing?
Any suggestion will be greatly appreciated!
Thanks !
I am gonna work on gene expression profiling in a non-model plant species, my advisor suggested using ScriptSeqTM Complete Kit*(plant) for sequencing libraries construction and then do a paired end sequencing(Supposed to be better than single end sequencing for the purpose of de novo assemble ?) using illumina Hiseq-2000.
Without a reference genome, I guess I will need either to use ref genome from a close related species or do a de novo assemble.
According to the library construction protocol, there is no size selection step for the final sequencing library, though they suggested to use bioanalyzer to estimate the size distribution in the library. So, my question is: will it be necessary to add a size selection step (200~400bp?) before sending out the library for a paired-end sequencing?
Any suggestion will be greatly appreciated!
Thanks !
Comment