Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation

Similar Threads
Thread Thread Starter Forum Replies Last Post
Library QC- cntrl loci enrichment doesn't match pre and post library generation yogioner Sample Prep / Library Generation 0 12-03-2012 04:40 PM
Enrich and Seq primers at RT overnight? gfpumpkins 454 Pyrosequencing 2 05-23-2012 08:54 AM
Can Truseq DNA library get >1ug DNA library at last? rnal Illumina/Solexa 2 04-12-2012 08:36 AM
Number of PCR How many PCR cycles to enrich adapter-modified DNA fragments MGH Man Sample Prep / Library Generation 5 07-26-2010 06:15 AM
Enrich the adaptor-modified DNA Fragments by PCR barbarella Illumina/Solexa 3 07-06-2009 07:43 AM

Thread Tools
Old 02-05-2013, 05:55 PM   #1
Location: philly

Join Date: Oct 2010
Posts: 17
Default can not get 1ug library to enrich

I am using trueseq kit to make library for enrichment, but I can always get only 400ng library, even I increase the input from 1ug to 5ug, the other thing is that whatever how much DNA was inputed, the final library is always about 400ng, the PCR cycles is always 10. where is the problem?
ychang is offline   Reply With Quote
Old 02-06-2013, 06:25 AM   #2
Senior Member
Location: Boston,MA

Join Date: Nov 2008
Posts: 122

Sounds as if your ligation is not correct or not efficient.

I would check your materials and concentrations for ligations.
kwaraska is offline   Reply With Quote

low library, truseq exome

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 07:38 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2022, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO