Let me preface this by saying I have never worked with an Ion torrent yet, but we just got an Ion S5, so I want to put it to good use.
Anyone know if I can ligate Ion torrent adapters during my library prep using Kapa Hyper Plus kit?
I want to use this kit:
Infographic here: https://sequencing.roche.com/content...-INFO-1216.pdf
Which uses A-Tailing for adapter ligation. And then I want to use Ion torrent Adapters instead of KAPA or Illumina adapters for use with Ion torrent. My question is:
are ion torrent adapters
(like these:https://www.thermofisher.com/order/c...rch-srp-A29751)
designed to work by A-tail ligation? you guys think I can just replace them? and then use primers for the P1 and A adapter sequences for the rest of the workflow? (Speaking of which, anyone know where I can find that info? Thermo does not seem to have made that readily available).
Any insight is much appreciated!
Anyone know if I can ligate Ion torrent adapters during my library prep using Kapa Hyper Plus kit?
I want to use this kit:
Infographic here: https://sequencing.roche.com/content...-INFO-1216.pdf
Which uses A-Tailing for adapter ligation. And then I want to use Ion torrent Adapters instead of KAPA or Illumina adapters for use with Ion torrent. My question is:
are ion torrent adapters
(like these:https://www.thermofisher.com/order/c...rch-srp-A29751)
designed to work by A-tail ligation? you guys think I can just replace them? and then use primers for the P1 and A adapter sequences for the rest of the workflow? (Speaking of which, anyone know where I can find that info? Thermo does not seem to have made that readily available).
Any insight is much appreciated!
Comment