Hi,
I was just wondering if anyone had any comments on whether there are advantages/disadvantages to merging fastq files from different sequencing runs of the same sample over merging the BAM files with regards alignment(using Tophat) ?
Also -for some of our samples we had sequencing runs with low read numbers 4-8 million reads (single end) & were advised not to merge these in with later runs for the same sample that had higher numbers so I was also wondering if anyone had any comments on that either as I'm not really sure why this is so.
Thanks in advance for any advice!!
I was just wondering if anyone had any comments on whether there are advantages/disadvantages to merging fastq files from different sequencing runs of the same sample over merging the BAM files with regards alignment(using Tophat) ?
Also -for some of our samples we had sequencing runs with low read numbers 4-8 million reads (single end) & were advised not to merge these in with later runs for the same sample that had higher numbers so I was also wondering if anyone had any comments on that either as I'm not really sure why this is so.
Thanks in advance for any advice!!
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