Has anyone experienced seeing a prominent non-rRNA peak after rRNA-depletion? I am seeing this consistently in my samples.
See the attached image for an example, which shows Bioanalyzer RNA pico before (top) and after (bottom) rRNA depletion by RiboZero.
These are bacterial RNA samples, so the main peaks in the total RNA are 23S and 16S (not 28S and 18S as annotated by the program). After rRNA depletion, the rRNA peaks are pretty much gone, but there is a very sharp, prominent peak running at about 400 nt.
Anyone have ideas on what this could be? It is just a very strongly expressed housekeeping gene? Or it it possibly something else?
See the attached image for an example, which shows Bioanalyzer RNA pico before (top) and after (bottom) rRNA depletion by RiboZero.
These are bacterial RNA samples, so the main peaks in the total RNA are 23S and 16S (not 28S and 18S as annotated by the program). After rRNA depletion, the rRNA peaks are pretty much gone, but there is a very sharp, prominent peak running at about 400 nt.
Anyone have ideas on what this could be? It is just a very strongly expressed housekeeping gene? Or it it possibly something else?
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