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Old 06-03-2011, 05:48 AM   #1
rteixeira
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Default single 18S pick

Good day.

I am analyzing plant small stem cross section for further Laser microdissection studies. Till now, all the samples shows a complete degradation of the RNA but i got a sample where i get a big 18Spick but no 28S. Does any one have encounter this kind of result?
Thank you so much
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Old 06-03-2011, 06:53 AM   #2
pbluescript
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Are you sure it is degradation? High salt buffer or sometimes organic contamination can make larger RNA bands insoluble so that they don't appear on a gel or bioanalyzer.
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Old 06-03-2011, 09:36 AM   #3
rteixeira
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Thank you so much for your reply. I am thinking in degradation because all my other samples came out as completely degraded using the same protocol. My hope is that is not degraded and something else occurred. i have added the graph.
Again, thank you so much for your help.
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File Type: pdf Eukaryote Total RNA Pico2011-05-2302.pdf (115.4 KB, 13 views)
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Old 06-03-2011, 09:49 AM   #4
pbluescript
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Hmm... that 18S peak looks a bit suspicious. Usually, such a sharp drop off on one side occurs when something went wrong with the run. How does the peak size compare to other samples?
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Old 06-03-2011, 10:02 AM   #5
rteixeira
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on other samples, the picks are very low. there is no comparison but i can send a graph or two. I will repeat the sample and see if that strange pick happens again.
this conversation has been a really helpful.
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