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Old 08-02-2012, 04:46 PM   #1
wvie
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Default Kmer Content

Hi,

What can be the cause of an increased number of 5-mers AAAAA and TTTTT?


Thank you.
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Old 08-10-2012, 05:11 PM   #2
Torst
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If you are sequencing repetitive regions of Human DNA for example, you would expect that.
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Old 08-10-2012, 11:38 PM   #3
Wallysb01
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Could be Poly-A tails too, if this is RNA-seq, or if you just didn't RNase your samples that well. Transcriptome assemblies have more absurd poly-A coverage than that though.
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Old 08-11-2012, 09:07 AM   #4
adaptivegenome
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Looks like polyA or lots of Alu sequences. What is the source of the data. This would help us debug.
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