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Old 05-13-2013, 09:26 AM   #1
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Location: Brazil

Join Date: May 2013
Posts: 1
Default Hello everyone!!

I'm a new research from Brazil who works with T. cacao, if someone likes chocolate so we have something in common. I'm writing a project to develop RAD-Seq markers and I'd like some helps on the budget, can someone help me??
ronaldocs1964 is offline   Reply With Quote
Old 05-13-2013, 10:56 AM   #2
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Location: Eugene, OR

Join Date: May 2013
Posts: 523

What do you want to know?
Do you know how many cut sites are in the genome? The number of RAD tags will be twice that. Are you planning single-end or paired-end sequencing? Do you want to capture heterozygosity at a high rate (higher read depth needed)?
For a single-end experiment, you might do a rough calculation like:
10 samples * 100,000 tags * 20X coverage * 30% "waste" = 26M reads.
Ordering adapters will add to the cost. Library consumables will be up to $20 per sample, depending on the project. Time... that is usually the driving cost factor, especially for trying it the first time. On both the library prep and analysis side of things.
SNPsaurus is offline   Reply With Quote

rad-seq, sequence data

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