Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation

Similar Threads
Thread Thread Starter Forum Replies Last Post
Automated Sciclone protocol for small RNA-Seq library prep is available Bioo Scientific Vendor Forum 0 09-01-2015 12:55 PM
Post-ChIP Sonication Protocol omy567 Epigenetics 5 02-12-2014 09:44 AM
Best protocol for checking size of sonicated chIP fragments??? Narelle Sample Prep / Library Generation 0 05-22-2013 06:49 PM

Thread Tools
Old 11-25-2015, 07:55 AM   #1
Junior Member
Location: Memphis, TN

Join Date: Mar 2015
Posts: 4
Default NEB ChIP protocol on Sciclone G3

Hi All,

We have had an increase in ChIP-seq recently and our protocol for library preparation is a bit kludgy (five total AMPure clean ups). Has anyone written or adapted a program to run on a Sciclone G3? We are unfortunately 'trapped' into using this protocol and are trying to find ways to make it less miserable. There is a similar TruSeq DNA protocol but the adaptor ligation and enrichment steps are different enough to make modification perilous.

Any thoughts, suggestions, or directions to written programs would be much appreciated.

jmhopkins is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 11:35 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO