Some times in colorspace mode bowtie reports quality values bigger than 4.0
Since, I think, this corresponds to probabilities bigger than 1
something has gone wrong.
The bowtie (0.12.9) manual says "the decoded nucleotide quality is either the sum of the overlapping color qualities....."
Should I simply divide the reported value by 2 ?
An example:
bowtie --seed 102509 -C Mycoplasma_allC
-1 SRR096735.21239778_1.filt.fastq
-2 SRR096735.21239778_2.filt.fastq
where SRR096735.21239778_1.filt.fastq contains:
@SRR096735.21239778 VAB_0312_20101203_1_SP_ANG_TG_NA20517_3_1sA_01003380619_497_712_492/1
T11222222222222222222222222222222222222222222222222
+
!=;@@=?<@>=?@>?@@A?@?@@??36>=@??A@>??@=???=<??>@<<>
SRR096735.21239778_2.filt.fastq contains:
@SRR096735.21239778 VAB_0312_20101203_1_SP_ANG_TG_NA20517_3_1sA_01003380619_497_712_492/2
T00122222222222222222222222222222222
+
!BB@ABBB?BABB6@?BB:9@BB9;?AB;9+>A<?;
The output is:
SRR096735.21239778 VAB_0312_20101203_1_SP_ANG_TG_NA20517_3_1sA_01003380619_497_712_492/1 + gi|339320528|ref|NC_015725.1| 187309 CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT Z_\[Z[]Z[^]\^_`_^^^_^]QHSZ\^]_`]\]^\[]][XZ]\][WY 1
SRR096735.21239778 VAB_0312_20101203_1_SP_ANG_TG_NA20517_3_1sA_01003380619_497_712_492/2 + gi|339320528|ref|NC_015725.1| 187326 ACTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT a`bcc``bbcWU^`c[RXacZSY_b\SCH^\ZY 1 0:T>A
Notice several quality values are Z (ascii 90, quality 5.7)
I am using 64bit bowtie 0.12.7 under linux
All comments and any help very welcome
Bill
Since, I think, this corresponds to probabilities bigger than 1
something has gone wrong.
The bowtie (0.12.9) manual says "the decoded nucleotide quality is either the sum of the overlapping color qualities....."
Should I simply divide the reported value by 2 ?
An example:
bowtie --seed 102509 -C Mycoplasma_allC
-1 SRR096735.21239778_1.filt.fastq
-2 SRR096735.21239778_2.filt.fastq
where SRR096735.21239778_1.filt.fastq contains:
@SRR096735.21239778 VAB_0312_20101203_1_SP_ANG_TG_NA20517_3_1sA_01003380619_497_712_492/1
T11222222222222222222222222222222222222222222222222
+
!=;@@=?<@>=?@>?@@A?@?@@??36>=@??A@>??@=???=<??>@<<>
SRR096735.21239778_2.filt.fastq contains:
@SRR096735.21239778 VAB_0312_20101203_1_SP_ANG_TG_NA20517_3_1sA_01003380619_497_712_492/2
T00122222222222222222222222222222222
+
!BB@ABBB?BABB6@?BB:9@BB9;?AB;9+>A<?;
The output is:
SRR096735.21239778 VAB_0312_20101203_1_SP_ANG_TG_NA20517_3_1sA_01003380619_497_712_492/1 + gi|339320528|ref|NC_015725.1| 187309 CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT Z_\[Z[]Z[^]\^_`_^^^_^]QHSZ\^]_`]\]^\[]][XZ]\][WY 1
SRR096735.21239778 VAB_0312_20101203_1_SP_ANG_TG_NA20517_3_1sA_01003380619_497_712_492/2 + gi|339320528|ref|NC_015725.1| 187326 ACTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT a`bcc``bbcWU^`c[RXacZSY_b\SCH^\ZY 1 0:T>A
Notice several quality values are Z (ascii 90, quality 5.7)
I am using 64bit bowtie 0.12.7 under linux
All comments and any help very welcome
Bill
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