Hello everyone!
I got my 100bp paired-end RNA-seq data today, FastQC told me that the duplication rate is above 60%. I searched around and found that it is common to get a high dup level with RNA-seq. Is that normal?
Should I remove the duplication? I heard some discussions in this forum that if the duplicates were removed then I cannot compare the highly expressed genes since the max depth of coverage at one point is 200 with 100bp sequencing data.
thanks!
I got my 100bp paired-end RNA-seq data today, FastQC told me that the duplication rate is above 60%. I searched around and found that it is common to get a high dup level with RNA-seq. Is that normal?
Should I remove the duplication? I heard some discussions in this forum that if the duplicates were removed then I cannot compare the highly expressed genes since the max depth of coverage at one point is 200 with 100bp sequencing data.
thanks!
Comment