I am using Nextera XT for library preparation.
The kit suggests 12 cycles of PCR after tagmentation.
If I run 5 ul of PCR product after 12 cycles I do not see any smear on my 1.5% agarose gel. If I run 13 cycles then I see the smear.
I am wondering if this is normal or if I should see a smear after 12 cycles.
I will try to go to the Bioanalyzer to see my library but I am not sure it will give me a better answer.
Any help or comment could be really useful.
The kit suggests 12 cycles of PCR after tagmentation.
If I run 5 ul of PCR product after 12 cycles I do not see any smear on my 1.5% agarose gel. If I run 13 cycles then I see the smear.
I am wondering if this is normal or if I should see a smear after 12 cycles.
I will try to go to the Bioanalyzer to see my library but I am not sure it will give me a better answer.
Any help or comment could be really useful.
Comment