Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation

Similar Threads
Thread Thread Starter Forum Replies Last Post
Improved ScriptMiner small-RNA-Seq kit now available epibio Vendor Forum 0 03-14-2012 12:28 PM
Help with using ScriptMiner small RNA library kit joytang1028 Sample Prep / Library Generation 0 02-17-2012 02:37 PM
ScriptMiner small RNA-seq library prep kit smalan RNA Sequencing 0 02-16-2012 05:02 AM
RNA-seq analysis --- TROUBLES--- vanale Illumina/Solexa 0 04-27-2010 05:21 AM

Thread Tools
Old 11-21-2012, 02:26 AM   #1
Junior Member
Location: Norway

Join Date: Aug 2012
Posts: 1
Default Scriptminer Small RNA-seq library troubles


I have a question regarding my small-RNA library results on my bioanalyzer.
Recording to the scriptminer protocol you should get a peak around 150 bp (including adapters and miRNA (18-40 nt).
Attached you find my bioanalyzer results on CD4 cells (RIN 9.0)
Library 1 is totRNA from manufacturer. THis looks just like it should be. The 3 next libraries is my CD4 human cells. Here i have DNA around 150 bp, but i also got a peak around 200 bp. Is this normal? Is it just larger rna fragments in these type of cells?
I also did a small-RNA library prep in wheat cells. Here my results showed a peak that had an area from 150 bp- 300 bp) Should i just go further with the rest of my samples?
Attached Files
File Type: pdf SKMBT_C36012112113090.pdf (229.4 KB, 30 views)
Helleaks is offline   Reply With Quote
Old 11-27-2012, 08:00 AM   #2
Senior Member
Location: MA

Join Date: Oct 2010
Posts: 160

Just a couple of questions; how do you get the Total/miRNA? I'm not sure about Scritpminer initial steps protocol, do you size select or use another kit (mirVana??).
The only way to know (I think) what you have in there is by colony screening (before sending to the Illumina machine); so you'll have an idea of potential contaminats in your library (rRNA...) and its proportion.
cascoamarillo is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 01:58 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO