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Hello guys, I am new in transcriptomic analysis. I am using tophat for mapping MACE reads (Massive analysis of 3' cDNA ends) in the wheat genome. I am analyzing salt-stressed and control samples. I have realized that some reads do map to chloroplast and mitochondrial sequences in the reference... and as far as i have understood the libraries preparation consider polyA capture. I think that I should keep this reads, but i am not really sure if its better only mapping to the nuclear genome. I want to hear more opinions, and advices and additional considerations about this issue, thank you very much for your help.
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM