Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • - N options with maq 0.7.1

    why -N option is not working? I am not able to print mismatch positions?

    Anyone has used - N options with maq 0.7.1? Please clarify whether this option is available. It always prints 0 at the last column of mapview results in text format. Thanks.

    When I execute ./maq map, I get the options as below:

    Usage: maq map [options] <out.map> <chr.bfa> <reads_1.bfq> [reads_2.bfq]
    Options: -1 INT length of the first read (<=127) [0]
    -2 INT length of the second read (<=127) [0]
    -m FLOAT rate of difference between reads and references [0.001]
    -e INT maximum allowed sum of qualities of mismatches [70]
    -d FILE adapter sequence file [null]
    -a INT max distance between two paired reads [250]
    -A INT max distance between two RF paired reads [0]
    -n INT number of mismatches in the first 24bp [2]
    -M c|g methylation alignment mode [null]
    -u FILE dump unmapped and poorly aligned reads to FILE [null]
    -H FILE dump multiple/all 01-mismatch hits to FILE [null]
    -C INT max number of hits to output. >512 for all 01 hits. [250]
    -s INT seed for random number generator [random]
    -W disable Smith-Waterman alignment
    -t trim all reads (usually not recommended)
    -c match in the colorspace
    Anyone knows that whether maq 0.7.1 has -N options working or do I have start using maq 0.6.8?

    Thanks.

  • #2
    I wonder how -e will influence the results? How much should I set for the 100pe reads.

    Comment

    Latest Articles

    Collapse

    • seqadmin
      Current Approaches to Protein Sequencing
      by seqadmin


      Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
      04-04-2024, 04:25 PM
    • seqadmin
      Strategies for Sequencing Challenging Samples
      by seqadmin


      Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
      03-22-2024, 06:39 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, 04-11-2024, 12:08 PM
    0 responses
    18 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-10-2024, 10:19 PM
    0 responses
    22 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-10-2024, 09:21 AM
    0 responses
    16 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-04-2024, 09:00 AM
    0 responses
    47 views
    0 likes
    Last Post seqadmin  
    Working...
    X