Hi all,
I am planning an experiment to look at DGE in a bacterial culture before and after nutrient depletion. We will be doing RNA-seq using an Illumina Hi-seq, and the facility has told us that they typically get 150-180 million reads/lane. I am wondering how many samples I can safely multiplex in one lane and still get enough depth to see differences in gene expression. I should have 4-6 time points in triplicate (12-18 samples). Any advice would be appreciated!
Thanks,
Sarah
I am planning an experiment to look at DGE in a bacterial culture before and after nutrient depletion. We will be doing RNA-seq using an Illumina Hi-seq, and the facility has told us that they typically get 150-180 million reads/lane. I am wondering how many samples I can safely multiplex in one lane and still get enough depth to see differences in gene expression. I should have 4-6 time points in triplicate (12-18 samples). Any advice would be appreciated!
Thanks,
Sarah
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