Apologies if this has been answered elsewhere, but I've had a glance through the threads and can't see anything obvious.
I've noted that Illumina's TruSeq kits are incompatible with BS-seq at present because the polymerase mix cannot tolerate uracil. My query is how much of an issue this is to sort out? Is it just a case of finding a different Taq for the library amplification (KAPA do one that seems suitable), or does this also affect the polymerases used to conduct end-repair? What about the polymerases in the SBS chemistry? We'd be wanting to prepare these for a run on a HiScanSQ.
Thanks,
Matt
I've noted that Illumina's TruSeq kits are incompatible with BS-seq at present because the polymerase mix cannot tolerate uracil. My query is how much of an issue this is to sort out? Is it just a case of finding a different Taq for the library amplification (KAPA do one that seems suitable), or does this also affect the polymerases used to conduct end-repair? What about the polymerases in the SBS chemistry? We'd be wanting to prepare these for a run on a HiScanSQ.
Thanks,
Matt
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