Hi,
Just wondering if anybody has tried to align subreads and build the CCS using alternative alignements programs, others than Blasr.
I´m thinking of bwa.
The problem is that if you are working with amplicon 16S data and don´t have a reference how would that work. So far bwa mem requires a reference database.
So anybody used alternative alignement programs to build consensus without a reference database ??
Just wondering if anybody has tried to align subreads and build the CCS using alternative alignements programs, others than Blasr.
I´m thinking of bwa.
The problem is that if you are working with amplicon 16S data and don´t have a reference how would that work. So far bwa mem requires a reference database.
So anybody used alternative alignement programs to build consensus without a reference database ??
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