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  • smallRNA v1.5 versus TruSEQ

    Hi all,

    I have sequenced the same small RNA sample using v1.5 or TruSEQ.
    As one might expect the data was overall similar, but what I have noticed is that RNAs that are 17-19 bp long (trimmed versions of mature microRNAs) were detected by v1.5 , but not by TruSEQ.

    So, I was wondering whether anybody knows the difference between these procedures that can explain the bias?

    Thanks.

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