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Old 11-24-2021, 09:01 PM   #1
Drmadscientist
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Location: India

Join Date: May 2014
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Default Weird Base Distribution in 10X DNA library

Hi Colleagues

I have been trying to sequence some 10X linked read DNA libraries from a plant for whole genome assembly. I am getting a weird pattern in the base distribution. We repeated this library a couple of times thinking that it was a library prep issue. Other sample libraries from different plants prepared along with this were fine. This was sequenced on Illumina Novaseq 6000. Can someone help explain this?

Thanks in Advance
MS
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10x, base composition, illumina, linked read

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