Hi,
I'm a PhD student and currently working on building up a Methylome of a plant species. I have a plant line which has been inbred for 9 generations to ensure homozygosity. I would like to know whether it is more robust to perform the sequencing from a) tissue of several individuals from the same mother pooled together and sequenced several times, b) tissue of several individuals from the same mother indexed then pooled, c) tissue of several individuals sequenced once each separately or d) tissue from a single individual sequenced several times?
* Using Illumina MiSeq *
Cheers,
Justin
I'm a PhD student and currently working on building up a Methylome of a plant species. I have a plant line which has been inbred for 9 generations to ensure homozygosity. I would like to know whether it is more robust to perform the sequencing from a) tissue of several individuals from the same mother pooled together and sequenced several times, b) tissue of several individuals from the same mother indexed then pooled, c) tissue of several individuals sequenced once each separately or d) tissue from a single individual sequenced several times?
* Using Illumina MiSeq *
Cheers,
Justin
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