Hi,
Has anyone got any experience in carrying out RNASeq from starting material with a RIN less than 8?
How well did it work?
We have some samples that continually fail to to give a RIN above 8, we are pretty sure it's because of the starting material we are extracting from, as others are fine. I'm pretty tempted to just give them a go and see how it turns out, but does anyone else have any experience of this?
How low can the RIN get before the sequencing is badly affected?
If anyone is willing to share their experiences it would be very much appreciated!
Thanks,
Jo
Has anyone got any experience in carrying out RNASeq from starting material with a RIN less than 8?
How well did it work?
We have some samples that continually fail to to give a RIN above 8, we are pretty sure it's because of the starting material we are extracting from, as others are fine. I'm pretty tempted to just give them a go and see how it turns out, but does anyone else have any experience of this?
How low can the RIN get before the sequencing is badly affected?
If anyone is willing to share their experiences it would be very much appreciated!
Thanks,
Jo
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