Does anyone out there strongly prefer either Nextera or standard (TruSeq) library construction for RNAseq?
I use a version of the standard protocol, which is familiar to me, not fast but automatable, cheap-ish, and comes with a bit of insert-end bias that I understand is due to bias in random hexamer synthesis or priming.
My sense is that the Nextera protocol is faster, more expensive in consumables ($80/sample for Illumina kit), and generates a longer region of insert-end bias which theoretically causes greater skewing of expression data.
Agree?
Disagree?
Why?
I use a version of the standard protocol, which is familiar to me, not fast but automatable, cheap-ish, and comes with a bit of insert-end bias that I understand is due to bias in random hexamer synthesis or priming.
My sense is that the Nextera protocol is faster, more expensive in consumables ($80/sample for Illumina kit), and generates a longer region of insert-end bias which theoretically causes greater skewing of expression data.
Agree?
Disagree?
Why?
Comment