I take 1 ug of total RNA and ribo deplete it. The leftover is usually 15 % of the total RNA. I am using reagent quantities as prescribed. May I ask how much Input you use??
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Your desired final concentration of your library will depend on what Illumina sequencer you're using and what the facility calls for. Will you be sequencing this library yourself?
For reference, Illumina recommends storing libraries at either 2nM (HiSeq/NextSeq) or 4nM (MiSeq) and diluting to the appropriate molarity before clustering. I generally store my libraries at >10nM as I see a gradual drop in molarity over time corresponding adsorption to the plastic storage tube -- this can be mitigated by using "LoBind" tubes and adding Tween to your storage buffer.
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Definitely a good idea to store at a higher concentration than needed for submission. For reference, you'll need to redilute your 151nM library to 10nM with e.g. 5uL of your library in 70.5uL of dilution buffer or whatever volumes work best for you. Illumina recommends Tris-Cl 10*mM, pH*8.5 with 0.1% Tween 20 rather than water.
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