Hi everyone =)
A colleague of mine advised me to create a de novo transcriptome assembly for my reads in sheep (because sheep annotations suck) but we couldn't find a way on the internet to do it for single-end reads. All of them were for paired-end and started by aligning Fw and Rv reads, so we're not quite sure how we should do it.
Have you done it before?
Cheers,
Seb.
A colleague of mine advised me to create a de novo transcriptome assembly for my reads in sheep (because sheep annotations suck) but we couldn't find a way on the internet to do it for single-end reads. All of them were for paired-end and started by aligning Fw and Rv reads, so we're not quite sure how we should do it.
Have you done it before?
Cheers,
Seb.
Comment