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Old 10-03-2019, 01:54 PM   #1
cement_head
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Default SMRT Link 7.0 - HGAP

Has anyone gotten the HGAP assembler to work? It seems that it just barfs at different places constantly. Doesn't seem to be very robust at all.

I've used CANU before, so I'm going to try to go back to that...

What about the wtdbg2assembler?

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My mistake - a corrupted downloaded data file was the culprit; should have checked the mdm5 checksum before starting!

Last edited by cement_head; 10-06-2019 at 07:12 AM. Reason: correction
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Old 10-03-2019, 02:34 PM   #2
SNPsaurus
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What kind of genome are you trying to assemble? I usually go with flye as a first pass (fast, memory efficient) and then Canu if flye underperforms (they seem to trade off which gives a better assembly in our hands). wtdbg2 is fun to try a quick check but I don't think it is as feature complete as flye or canu.
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Old 10-06-2019, 07:09 AM   #3
cement_head
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Cool

Quote:
Originally Posted by SNPsaurus View Post
What kind of genome are you trying to assemble? I usually go with flye as a first pass (fast, memory efficient) and then Canu if flye underperforms (they seem to trade off which gives a better assembly in our hands). wtdbg2 is fun to try a quick check but I don't think it is as feature complete as flye or canu.
Well, figured it out - was a corrupted file from the download!

Wood Frog Genome - 6 Gbp

Got it working, but it barfs as it wants to fo a 30x coverage and from the RAW reads, it comes up a few thousand short. Would the SEED COVERAGE parameter (in the Advanced tab) be the one I would want to change? From say 30 to 25? (Just to get a rough assembly?) I'm waiting on HiSeq data to do a combined ONT + Pac Bio + HiSeq assembly in CANU.

Last edited by cement_head; 10-06-2019 at 07:10 AM. Reason: clarification
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Old 10-06-2019, 10:08 AM   #4
SNPsaurus
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Oh my, that's a big one. How much memory is it using?

If you just want a rough assembly, I would do wtdbg2 as you can get a sense of contig lengths without consensus generation. I've done flye with 10X read coverage (to look at how much is chloroplast and bacteria in a dirty sample) and it didn't protest. Sorry, haven't used HGAP.
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