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Old 06-27-2011, 10:07 AM   #1
hfaoro
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Location: Curitiba, Brazil

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Default Changing the read length after the run had started

Hellow,
I started a transcriptome barcoding run in SOLiD4. After the barcoding sequencing was finish I realized that the read length for tag F3 was 5 bp instead of 50 bp. I would like to know if someone had this problem and if is possible to change the read length in this stage.
Thanks all.
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Old 06-27-2011, 12:18 PM   #2
pmiguel
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Location: Purdue University, West Lafayette, Indiana

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Yes, but it is not trivial. We had this happen once. Applied Biosystems had a Bioinformatics Field Specialist remotely modify the run to the correct number of bases.

Not a minor undertaking, though.

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Phillip
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