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06-02-2013, 12:53 AM
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#1 |
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Junior Member
Location: - Join Date: Jun 2013
Posts: 9
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Help to plan experiment
Hello everyone,
I am a biology student and I need to check the microbial population in the intestine before and after taking a certain medication, to see if the drug affects the microbe abundance in the intestine... I need to plan this experiment using NGS and I am LOST! please can someone help me to plan this experiment...? what NGS technology should I use after extraction of DNA/RNA samples? Thank You! |
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06-03-2013, 04:47 AM
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#2 |
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Senior Member
Location: New England Join Date: Jun 2012
Posts: 200
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Have you looked into 16s rDNA sequencing? It may not be the best solution, but there are lots of papers out there using this method.
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06-03-2013, 05:36 AM
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#3 |
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Senior Member
Location: sub-surface moon base Join Date: Apr 2013
Posts: 372
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1. Think very hard what biological questions you want to address
2. Find out how much funding you have 3. Read a ton of papers 4. Make an informed decision |
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