Go Back   SEQanswers > Sequencing Technologies/Companies > Illumina/Solexa
Similar Threads
Thread Thread Starter Forum Replies Last Post
Experiment deletion failing BenjaminL Ion Torrent 0 07-29-2012 10:10 PM
Need Help Designing Experiment. master_shake RNA Sequencing 0 07-17-2012 01:56 PM
How many tags to perform a RNAseq experiment kant1_IC RNA Sequencing 6 02-06-2012 06:18 AM
Any plan to upgrade the backend system? marcowanger Site Feedback/Suggestions 0 11-03-2011 12:23 AM
Minimum information about NGS Experiment wdt Bioinformatics 3 03-21-2011 12:24 PM

Thread Tools
Old 06-02-2013, 12:53 AM   #1
Junior Member
Location: -

Join Date: Jun 2013
Posts: 9
Default Help to plan experiment

Hello everyone,

I am a biology student and I need to check the microbial population in the intestine before and after taking a certain medication, to see if the drug affects the microbe abundance in the intestine... I need to plan this experiment using NGS and I am LOST!
please can someone help me to plan this experiment...? what NGS technology should I use after extraction of DNA/RNA samples?

Thank You!
Nullie is offline   Reply With Quote
Old 06-03-2013, 04:47 AM   #2
Senior Member
Location: New England

Join Date: Jun 2012
Posts: 200

Have you looked into 16s rDNA sequencing? It may not be the best solution, but there are lots of papers out there using this method.
microgirl123 is offline   Reply With Quote
Old 06-03-2013, 05:36 AM   #3
Senior Member
Location: sub-surface moon base

Join Date: Apr 2013
Posts: 372

1. Think very hard what biological questions you want to address
2. Find out how much funding you have
3. Read a ton of papers
4. Make an informed decision
rhinoceros is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 07:31 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2022, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO