Hello,
Anyone who has experience with demultiplexing Sequel data via SMRT link command line options?
What I've done so far is to follow the "Barcode splitting for command-line analysis (SMRT Analysis v3.1)" section in
and then use the demultiplex.py at https://github.com/PacificBiosciences/SMRT-Link
But when I do assembly with the demultiplexed data the results I get are just strange and not comparable to the assembly results I get from the same machine without demultiplexing. So I'm guessing that I'm doing something wrong.
Thanks,
Ali
Anyone who has experience with demultiplexing Sequel data via SMRT link command line options?
What I've done so far is to follow the "Barcode splitting for command-line analysis (SMRT Analysis v3.1)" section in
and then use the demultiplex.py at https://github.com/PacificBiosciences/SMRT-Link
But when I do assembly with the demultiplexed data the results I get are just strange and not comparable to the assembly results I get from the same machine without demultiplexing. So I'm guessing that I'm doing something wrong.
Thanks,
Ali
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