I am hoping someone else has encountered this issue:
We have shotgun metagenomic samples which we have blasted against nr ( downloaded dec 2014); when we import the blast output onto MEGAN it all looks fine at the top taxonomical level.
However when we try to look at KEGG or COG we get zero reads assigned; this happens even when I load the Auxiliary mapping files for KEGG analysis as suggested on the MEGAN website. ( at the Load GI mapping tab on the KEGG tab)
Am I missing something completely obvious?
Orla
We have shotgun metagenomic samples which we have blasted against nr ( downloaded dec 2014); when we import the blast output onto MEGAN it all looks fine at the top taxonomical level.
However when we try to look at KEGG or COG we get zero reads assigned; this happens even when I load the Auxiliary mapping files for KEGG analysis as suggested on the MEGAN website. ( at the Load GI mapping tab on the KEGG tab)
Am I missing something completely obvious?
Orla
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