Is short-read sequencing a haploid or diploid sequencing?
If it is a diploid sequencing, does the read containing a heterozygous indel show similar pattern as Sanger sequencing (mixed peaks on tail)? For example, mixed color dots on the later cycles in illumina sequencing?
If it is a diploid sequencing, does the read containing a heterozygous indel show similar pattern as Sanger sequencing (mixed peaks on tail)? For example, mixed color dots on the later cycles in illumina sequencing?
Comment